Used methods and devices
xMAP technology - multiplex detection of swine cytokines (IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12p40, TNF-alpha and IFN-gamma); Bio-Plex/Luminex (Bio-Rad).
RT-qPCR - transtcipts related to intestinal barrier, Toll- and Nod-like receptors, Universal Probe Library (Roche Diagnostics), since 2022 based on SYBR Green system; Tower G3 (Analytik Jena).
ELISA - swine IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12/23 p40, IL-18, TNF-alpha, IFN-gamma, CRP, HMGB1; Infinite M200 (Tecan).
IHC - HMGB1, RAGE, TLR5; BX 40 microscope (Olympus), EVOS FL (Life Technologies).
IF - claudin 1, occludin, ZO-1, HMGB1; BX 40 microscope (Olympus, Tokyo, Japan), EVOS FL (Life Technologies).
Others - Western blotting, histology, hybridoma biotechnology, classical bacteriology.