GNOTOBIO

Biomedical research

Used methods and devices

xMAPmultiplex detection of swine cytokines (IL-1beta, IL-4,  IL-6, IL-8, IL-10, IL-12p40, TNF-alpha and IFN-gamma); Bio-Plex/Luminex (Bio-Rad, Hercules, USA).

RT-qPCR - wide penel of transtcipts by Universal Probe Library (Roche Diagnostics); iQ5( Bio-Rad, Hercules, USA). 

ELISA - swine IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12/23 p40, IL-18, TNF-alpha, IFN-gamma, CRP, HMGB1; Infinite M200 (Tecan, Grodung, USA).

IHC - HMGB1, RAGE, TLR5; BX 40 microscope (Olympus, Tokyo, Japan), EVOS FL (Life Technologies, Carlsbad, CA). 

IF - claudin 1, occludin, ZO-1, HMGB1; BX 40 microscope (Olympus, Tokyo, Japan), EVOS FL (Life Technologies, Carlsbad, CA). 

Others - Western blotting, histology, hybridoma biotechnology, classical bacteriology.